Substrate-level phosphorylation in isolated yeast mitochondria

Oxidative phosphorylation in cardiolipin-lacking yeast mitochondria.

The role of cardiolipin in mitochondrial energy transformation was studied by comparing oxidative phosphorylation in the Saccharomyces cerevisiae cardiolipin synthase null mutant crd1Delta, and in isogenic wild type. Oxygen consumption experiments and membrane potential kinetics during the phosphorylation cycle in isolated mitochondria indicated that the absence of cardiolipin causes only a mod...

Control of phosphoenolpyruvate synthesis by substrate level phosphorylation in guinea pig liver mitochondria.

Control of P-enolpyruvate synthesis has been studied in guinea pig liver mitochondria with respect to the relative rates of GTP generation directly from substrate level phosphorylation or indirectly from ATP via nucleoside diphosphate kinase. With malate or pyruvate plus malate as substrates, P-enolpyruvate production was greatly decreased in State 3 by addition of fluorocitrate (an inhibitor o...

Increasing mitochondrial substrate-level phosphorylation can rescue respiratory growth of an ATP synthase-deficient yeast.

In a previous study we have identified Fmc1p, a mitochondrial protein involved in the assembly/stability of the yeast F0F1-ATP synthase at elevated temperatures. The deltafmc1 mutant was shown to exhibit a severe phenotype of very slow growth on respiratory substrates at 37 degrees C. We have isolated ODC1 as a multicopy suppressor of the fmc1 deletion restoring a good respiratory growth. Odc1p...

Oxidative phosphorylation in mitochondria isolated from chronically stressed dog hearts.

Oxidative and phosphorylative activities were measured polarographically in mitochondria isolated from the right and left ventricles of normal and chronically stressed dog hearts. Chronic myocardial stress was produced experimentally by surgical procedures (combined tricuspid insufficiency and pulmonary stenosis, pulmonary insufficiency, aortic stenosis, aortic insufficiency, Potts's anastomosi...

Modelling of oxidative phosphorylation in isolated mitochondria and intact cells